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golph3 polyclonal antibody  (Proteintech)


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    Structured Review

    Proteintech golph3 polyclonal antibody
    Golph3 Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/golph3+polyclonal+antibody/GOLPH3+Antibody/pmc12969053-25-0-4
    Average 94 stars, based on 38 article reviews
    golph3 polyclonal antibody - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Expressing:

    Article Title: DNA-PKcs controls the cytotoxic T cell response to cancer and transplant allograft through regulating LAT-dependent signaling
    Article Snippet: CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.. GOLPH3 Polyclonal antibody , Proteintech , Cat#19112: RRID: AB_2113342.. CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.

    Article Title: Circular GOLPH3 RNA exerts oncogenic effects in vitro by regulating the miRNA-1299/LIF axis in oral squamous cell carcinoma
    Article Snippet: The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.. The primary antibodies used were: LIF (ab172023, abcam, Cambridge, MA, USA), GOLPH3 Polyclonal antibody (Proteintech Group, Chicago, IL, USA).. The bands were measured by ECL western blot kit [ ].The bands were measured by ECL western blot kit [ ].

    Fluorescence:

    Article Title: DNA-PKcs controls the cytotoxic T cell response to cancer and transplant allograft through regulating LAT-dependent signaling
    Article Snippet: CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.. GOLPH3 Polyclonal antibody , Proteintech , Cat#19112: RRID: AB_2113342.. CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.

    Article Title: Circular GOLPH3 RNA exerts oncogenic effects in vitro by regulating the miRNA-1299/LIF axis in oral squamous cell carcinoma
    Article Snippet: The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.. The primary antibodies used were: LIF (ab172023, abcam, Cambridge, MA, USA), GOLPH3 Polyclonal antibody (Proteintech Group, Chicago, IL, USA).. The bands were measured by ECL western blot kit [ ].The bands were measured by ECL western blot kit [ ].

    In Situ Hybridization:

    Article Title: DNA-PKcs controls the cytotoxic T cell response to cancer and transplant allograft through regulating LAT-dependent signaling
    Article Snippet: CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.. GOLPH3 Polyclonal antibody , Proteintech , Cat#19112: RRID: AB_2113342.. CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.

    Article Title: Circular GOLPH3 RNA exerts oncogenic effects in vitro by regulating the miRNA-1299/LIF axis in oral squamous cell carcinoma
    Article Snippet: The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.. The primary antibodies used were: LIF (ab172023, abcam, Cambridge, MA, USA), GOLPH3 Polyclonal antibody (Proteintech Group, Chicago, IL, USA).. The bands were measured by ECL western blot kit [ ].The bands were measured by ECL western blot kit [ ].

    Fractionation:

    Article Title: DNA-PKcs controls the cytotoxic T cell response to cancer and transplant allograft through regulating LAT-dependent signaling
    Article Snippet: CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.. GOLPH3 Polyclonal antibody , Proteintech , Cat#19112: RRID: AB_2113342.. CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.

    Article Title: Circular GOLPH3 RNA exerts oncogenic effects in vitro by regulating the miRNA-1299/LIF axis in oral squamous cell carcinoma
    Article Snippet: The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.. The primary antibodies used were: LIF (ab172023, abcam, Cambridge, MA, USA), GOLPH3 Polyclonal antibody (Proteintech Group, Chicago, IL, USA).. The bands were measured by ECL western blot kit [ ].The bands were measured by ECL western blot kit [ ].

    Real-time Polymerase Chain Reaction:

    Article Title: DNA-PKcs controls the cytotoxic T cell response to cancer and transplant allograft through regulating LAT-dependent signaling
    Article Snippet: CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.. GOLPH3 Polyclonal antibody , Proteintech , Cat#19112: RRID: AB_2113342.. CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.

    Article Title: Circular GOLPH3 RNA exerts oncogenic effects in vitro by regulating the miRNA-1299/LIF axis in oral squamous cell carcinoma
    Article Snippet: The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.. The primary antibodies used were: LIF (ab172023, abcam, Cambridge, MA, USA), GOLPH3 Polyclonal antibody (Proteintech Group, Chicago, IL, USA).. The bands were measured by ECL western blot kit [ ].The bands were measured by ECL western blot kit [ ].

    Quantitative RT-PCR:

    Article Title: DNA-PKcs controls the cytotoxic T cell response to cancer and transplant allograft through regulating LAT-dependent signaling
    Article Snippet: CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.. GOLPH3 Polyclonal antibody , Proteintech , Cat#19112: RRID: AB_2113342.. CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.

    Article Title: Circular GOLPH3 RNA exerts oncogenic effects in vitro by regulating the miRNA-1299/LIF axis in oral squamous cell carcinoma
    Article Snippet: The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.. The primary antibodies used were: LIF (ab172023, abcam, Cambridge, MA, USA), GOLPH3 Polyclonal antibody (Proteintech Group, Chicago, IL, USA).. The bands were measured by ECL western blot kit [ ].The bands were measured by ECL western blot kit [ ].

    Isolation:

    Article Title: DNA-PKcs controls the cytotoxic T cell response to cancer and transplant allograft through regulating LAT-dependent signaling
    Article Snippet: CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.. GOLPH3 Polyclonal antibody , Proteintech , Cat#19112: RRID: AB_2113342.. CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.

    Article Title: Circular GOLPH3 RNA exerts oncogenic effects in vitro by regulating the miRNA-1299/LIF axis in oral squamous cell carcinoma
    Article Snippet: The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.. The primary antibodies used were: LIF (ab172023, abcam, Cambridge, MA, USA), GOLPH3 Polyclonal antibody (Proteintech Group, Chicago, IL, USA).. The bands were measured by ECL western blot kit [ ].The bands were measured by ECL western blot kit [ ].

    Migration:

    Article Title: DNA-PKcs controls the cytotoxic T cell response to cancer and transplant allograft through regulating LAT-dependent signaling
    Article Snippet: CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.. GOLPH3 Polyclonal antibody , Proteintech , Cat#19112: RRID: AB_2113342.. CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.

    Article Title: Circular GOLPH3 RNA exerts oncogenic effects in vitro by regulating the miRNA-1299/LIF axis in oral squamous cell carcinoma
    Article Snippet: The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.. The primary antibodies used were: LIF (ab172023, abcam, Cambridge, MA, USA), GOLPH3 Polyclonal antibody (Proteintech Group, Chicago, IL, USA).. The bands were measured by ECL western blot kit [ ].The bands were measured by ECL western blot kit [ ].

    Transfection:

    Article Title: DNA-PKcs controls the cytotoxic T cell response to cancer and transplant allograft through regulating LAT-dependent signaling
    Article Snippet: CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.. GOLPH3 Polyclonal antibody , Proteintech , Cat#19112: RRID: AB_2113342.. CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.

    Article Title: Circular GOLPH3 RNA exerts oncogenic effects in vitro by regulating the miRNA-1299/LIF axis in oral squamous cell carcinoma
    Article Snippet: The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.. The primary antibodies used were: LIF (ab172023, abcam, Cambridge, MA, USA), GOLPH3 Polyclonal antibody (Proteintech Group, Chicago, IL, USA).. The bands were measured by ECL western blot kit [ ].The bands were measured by ECL western blot kit [ ].

    Knockdown:

    Article Title: DNA-PKcs controls the cytotoxic T cell response to cancer and transplant allograft through regulating LAT-dependent signaling
    Article Snippet: CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.. GOLPH3 Polyclonal antibody , Proteintech , Cat#19112: RRID: AB_2113342.. CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.

    Article Title: Circular GOLPH3 RNA exerts oncogenic effects in vitro by regulating the miRNA-1299/LIF axis in oral squamous cell carcinoma
    Article Snippet: The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.. The primary antibodies used were: LIF (ab172023, abcam, Cambridge, MA, USA), GOLPH3 Polyclonal antibody (Proteintech Group, Chicago, IL, USA).. The bands were measured by ECL western blot kit [ ].The bands were measured by ECL western blot kit [ ].

    Over Expression:

    Article Title: DNA-PKcs controls the cytotoxic T cell response to cancer and transplant allograft through regulating LAT-dependent signaling
    Article Snippet: CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.. GOLPH3 Polyclonal antibody , Proteintech , Cat#19112: RRID: AB_2113342.. CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.

    Article Title: Circular GOLPH3 RNA exerts oncogenic effects in vitro by regulating the miRNA-1299/LIF axis in oral squamous cell carcinoma
    Article Snippet: The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.. The primary antibodies used were: LIF (ab172023, abcam, Cambridge, MA, USA), GOLPH3 Polyclonal antibody (Proteintech Group, Chicago, IL, USA).. The bands were measured by ECL western blot kit [ ].The bands were measured by ECL western blot kit [ ].

    Plasmid Preparation:

    Article Title: DNA-PKcs controls the cytotoxic T cell response to cancer and transplant allograft through regulating LAT-dependent signaling
    Article Snippet: CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.. GOLPH3 Polyclonal antibody , Proteintech , Cat#19112: RRID: AB_2113342.. CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.

    Article Title: Circular GOLPH3 RNA exerts oncogenic effects in vitro by regulating the miRNA-1299/LIF axis in oral squamous cell carcinoma
    Article Snippet: The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.. The primary antibodies used were: LIF (ab172023, abcam, Cambridge, MA, USA), GOLPH3 Polyclonal antibody (Proteintech Group, Chicago, IL, USA).. The bands were measured by ECL western blot kit [ ].The bands were measured by ECL western blot kit [ ].

    Cell Counting:

    Article Title: DNA-PKcs controls the cytotoxic T cell response to cancer and transplant allograft through regulating LAT-dependent signaling
    Article Snippet: CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.. GOLPH3 Polyclonal antibody , Proteintech , Cat#19112: RRID: AB_2113342.. CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.

    Article Title: Circular GOLPH3 RNA exerts oncogenic effects in vitro by regulating the miRNA-1299/LIF axis in oral squamous cell carcinoma
    Article Snippet: The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.. The primary antibodies used were: LIF (ab172023, abcam, Cambridge, MA, USA), GOLPH3 Polyclonal antibody (Proteintech Group, Chicago, IL, USA).. The bands were measured by ECL western blot kit [ ].The bands were measured by ECL western blot kit [ ].

    Invasion Assay:

    Article Title: DNA-PKcs controls the cytotoxic T cell response to cancer and transplant allograft through regulating LAT-dependent signaling
    Article Snippet: CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.. GOLPH3 Polyclonal antibody , Proteintech , Cat#19112: RRID: AB_2113342.. CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.

    Article Title: Circular GOLPH3 RNA exerts oncogenic effects in vitro by regulating the miRNA-1299/LIF axis in oral squamous cell carcinoma
    Article Snippet: The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.. The primary antibodies used were: LIF (ab172023, abcam, Cambridge, MA, USA), GOLPH3 Polyclonal antibody (Proteintech Group, Chicago, IL, USA).. The bands were measured by ECL western blot kit [ ].The bands were measured by ECL western blot kit [ ].

    Mutagenesis:

    Article Title: DNA-PKcs controls the cytotoxic T cell response to cancer and transplant allograft through regulating LAT-dependent signaling
    Article Snippet: CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.. GOLPH3 Polyclonal antibody , Proteintech , Cat#19112: RRID: AB_2113342.. CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.

    Article Title: Circular GOLPH3 RNA exerts oncogenic effects in vitro by regulating the miRNA-1299/LIF axis in oral squamous cell carcinoma
    Article Snippet: The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.. The primary antibodies used were: LIF (ab172023, abcam, Cambridge, MA, USA), GOLPH3 Polyclonal antibody (Proteintech Group, Chicago, IL, USA).. The bands were measured by ECL western blot kit [ ].The bands were measured by ECL western blot kit [ ].

    Binding Assay:

    Article Title: DNA-PKcs controls the cytotoxic T cell response to cancer and transplant allograft through regulating LAT-dependent signaling
    Article Snippet: CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.. GOLPH3 Polyclonal antibody , Proteintech , Cat#19112: RRID: AB_2113342.. CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.

    Article Title: Circular GOLPH3 RNA exerts oncogenic effects in vitro by regulating the miRNA-1299/LIF axis in oral squamous cell carcinoma
    Article Snippet: The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.. The primary antibodies used were: LIF (ab172023, abcam, Cambridge, MA, USA), GOLPH3 Polyclonal antibody (Proteintech Group, Chicago, IL, USA).. The bands were measured by ECL western blot kit [ ].The bands were measured by ECL western blot kit [ ].

    Luciferase:

    Article Title: DNA-PKcs controls the cytotoxic T cell response to cancer and transplant allograft through regulating LAT-dependent signaling
    Article Snippet: CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.. GOLPH3 Polyclonal antibody , Proteintech , Cat#19112: RRID: AB_2113342.. CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.

    Article Title: Circular GOLPH3 RNA exerts oncogenic effects in vitro by regulating the miRNA-1299/LIF axis in oral squamous cell carcinoma
    Article Snippet: The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.. The primary antibodies used were: LIF (ab172023, abcam, Cambridge, MA, USA), GOLPH3 Polyclonal antibody (Proteintech Group, Chicago, IL, USA).. The bands were measured by ECL western blot kit [ ].The bands were measured by ECL western blot kit [ ].

    CCK-8 Assay:

    Article Title: DNA-PKcs controls the cytotoxic T cell response to cancer and transplant allograft through regulating LAT-dependent signaling
    Article Snippet: CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.CLINT1 Polyclonal antibody , Proteintech , Cat#10470; RRID: AB_2276405.. GOLPH3 Polyclonal antibody , Proteintech , Cat#19112: RRID: AB_2113342.. CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.CD3z Monoclonal Antibody (4B10) , Invitrogen , Cat#ma5-15608; RRID: AB_10986021.

    Article Title: Circular GOLPH3 RNA exerts oncogenic effects in vitro by regulating the miRNA-1299/LIF axis in oral squamous cell carcinoma
    Article Snippet: The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.The NC membranes were incubated with primary antibody for 12 hours at 4°C, and with the second antibody for 2 hours at 37°C.. The primary antibodies used were: LIF (ab172023, abcam, Cambridge, MA, USA), GOLPH3 Polyclonal antibody (Proteintech Group, Chicago, IL, USA).. The bands were measured by ECL western blot kit [ ].The bands were measured by ECL western blot kit [ ].



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    3OST5 interacts with <t>GOLPH3.</t> EC‐3OST5 cell lysate was immunoprecipitated for GFP using the anti‐GFP antibody and revealed for the GOLPH3 protein. The upper membrane was incubated with the primary antibody against GOLPH3 (1 : 1000) and detected using an anti‐rabbit HRP‐linked secondary antibody (1 : 2500). Meanwhile, the lower membrane was developed using only an anti‐goat HRP‐linked secondary antibody (1 : 2500) and, as a result, the band corresponding to the 3OST5‐GFP protein in the input lane is absent. The data reflect the outcomes of two independent experiments.
    Rabbit Polyclonal Anti Golph3, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Danaher Inc rabbit polyclonal antibody to golph3
    3OST5 interacts with <t>GOLPH3.</t> EC‐3OST5 cell lysate was immunoprecipitated for GFP using the anti‐GFP antibody and revealed for the GOLPH3 protein. The upper membrane was incubated with the primary antibody against GOLPH3 (1 : 1000) and detected using an anti‐rabbit HRP‐linked secondary antibody (1 : 2500). Meanwhile, the lower membrane was developed using only an anti‐goat HRP‐linked secondary antibody (1 : 2500) and, as a result, the band corresponding to the 3OST5‐GFP protein in the input lane is absent. The data reflect the outcomes of two independent experiments.
    Rabbit Polyclonal Antibody To Golph3, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    3OST5 interacts with GOLPH3. EC‐3OST5 cell lysate was immunoprecipitated for GFP using the anti‐GFP antibody and revealed for the GOLPH3 protein. The upper membrane was incubated with the primary antibody against GOLPH3 (1 : 1000) and detected using an anti‐rabbit HRP‐linked secondary antibody (1 : 2500). Meanwhile, the lower membrane was developed using only an anti‐goat HRP‐linked secondary antibody (1 : 2500) and, as a result, the band corresponding to the 3OST5‐GFP protein in the input lane is absent. The data reflect the outcomes of two independent experiments.

    Journal: The Febs Journal

    Article Title: Involvement of GTPases and vesicle adapter proteins in Heparan sulfate biosynthesis: role of Rab1A , Rab2A and GOLPH3

    doi: 10.1111/febs.17398

    Figure Lengend Snippet: 3OST5 interacts with GOLPH3. EC‐3OST5 cell lysate was immunoprecipitated for GFP using the anti‐GFP antibody and revealed for the GOLPH3 protein. The upper membrane was incubated with the primary antibody against GOLPH3 (1 : 1000) and detected using an anti‐rabbit HRP‐linked secondary antibody (1 : 2500). Meanwhile, the lower membrane was developed using only an anti‐goat HRP‐linked secondary antibody (1 : 2500) and, as a result, the band corresponding to the 3OST5‐GFP protein in the input lane is absent. The data reflect the outcomes of two independent experiments.

    Article Snippet: Rabbit antibodies against α‐COP, β‐COP, GM130, Calreticulin and Syndecan‐3 were purchased from Abcam, antibodies against 3OST5 and COPII from Thermo Scientific (Rockford, IL, USA), antibody to 2OST (N‐term) from Abgent, antibodies against Rab1A and GOLPH3 from Origene and antibodies against 6OST1, Syndecan‐2 and Syndecan‐4 from Santa Cruz Biotechnology (Dallas, TX, USA).

    Techniques: Immunoprecipitation, Membrane, Incubation